4.2 Article

A fast, aqueous, reversible three-day tissue clearing method for adult and embryonic mouse brain and whole body

Journal

CELL REPORTS METHODS
Volume 1, Issue 7, Pages -

Publisher

CELL PRESS
DOI: 10.1016/j.crmeth.2021.100090

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Funding

  1. Howard Hughes Medical Institute

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Fast 3D Clear is an aqueous clearing protocol that can achieve high transparency of adult and embryonic mouse tissues in a few days, making it suitable for a wide range of biomedical studies.
Optical clearing methods serve as powerful tools to study intact organs and neuronal circuits. We developed an aqueous clearing protocol, Fast 3D Clear, that relies on tetrahydrofuran for tissue delipidation and iohexol for clearing, such that tissues can be imaged under immersion oil in light-sheet imaging systems. Fast 3D Clear requires 3 days to achieve high transparency of adult and embryonic mouse tissues while maintaining their anatomical integrity and preserving a vast array of transgenic and viral/dye fluorophores. A unique advantage of Fast 3D Clear is its complete reversibility and thus compatibility with tissue sectioning and immunohistochemistry. Fast 3D Clear can be easily and quickly applied to a wide range of biomedical studies, facilitating the acquisition of high-resolution two- and three- dimensional images.

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