Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 85, Issue 11, Pages 2221-2223Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bbb/zbab148
Keywords
D-alanine assay; alanine racemase; l-alanine dehydrogenase; crustacean
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An enzymatic assay system was constructed to determine the D-Ala contents of 7 crustaceans, which involves the conversion of D-Ala to L-Ala by alanine racemase and subsequent deamination by L-alanine dehydrogenase with the reduction of NAD(+) to NADH.
An enzymatic assay system of D-Ala, which is reported to affect the taste, was constructed using alanine racemase and L-alanine dehydrogenase. D-Ala is converted to L-Ala by alanine racemase and then deaminated by L-alanine dehydrogenase with the reduction of NAD(+) to NADH, which is determined with water-soluble tetrazolium. Using the assay system, the D-Ala contents of 7 crustaceans were determined.
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