Identification and characterization of two highly homologous lysozymes from red swamp crayfish, Procambarus clarkii

Lysozyme is an important immune effector in innate immunity against pathogen infection. But the study on the active region of lysozyme is limited. In this study, two highly homologous lysozymes were identified from crayfish (designated as PcLysi4 and PcLysi5). The molecular structures of PcLysi4 and PcLysi5 were predicted by SWISS-MODEL with the structure of lysozyme (PDB accession No. 4PJ2.2.B) as model. The results suggested that the structure of PcLysi4 and PcLysi5 were highly similar, but there were more a-helices at positions (127-139) and longer beta-sheet at positions (49-57) in the structure of PcLysi5 than in that of PcLysi4. The antibacterial and antiviral functions of the two lysozymes were investigated. PcLysi4 and PcLysi5 could promote the bacterial clearance ability of crayfish, and increase the survival rate of Vibrio-infected crayfish. Further study showed that PcLysi5 inhibited WSSV replication, and enhanced the survival rate of WSSV-infected crayfish. There was no evidence that PcLysi4 has an influence on WSSV replication. Furthermore, PcLysi5 was detected to interact with envelope protein VP24 of WSSV. Our results would provide a new reference for the study on active region of lysozyme.

Automated summary

In this study, two highly homologous lysozymes (PcLysi4 and PcLysi5) were identified from crayfish. The structure of PcLysi4 and PcLysi5 showed differences in α-helices and beta-sheets. Both lysozymes were found to enhance bacterial clearance ability and increase survival rate in Vibrio-infected crayfish, with PcLysi5 also inhibiting WSSV replication and interacting with envelope protein VP24. These findings provide new insights into the active region of lysozyme.