Identifying active rumen epithelial associated bacteria and archaea in beef cattle divergent in feed efficiency using total RNA-seq

To date, the role of ruminal epithelial attached microbiota in cattle feed efficiency is undefined. In this study, we aimed to characterize transcriptionally active bacteria and archaea attached to the rumen epithelial wall and to determine whether they differ in cattle with varied feed efficiency. RNA-sequencing was performed to obtain the rumen epithelial transcriptomes from 9 of the most efficient (low RFI) and 9 of the most inefficient (high RFI) animals. The bacteria and archaea 16S rRNA transcripts were identified using an in-house developed pipeline, enriched from filtered reads that did not map to the bovine genome. Archaea from unclassified genera belonging to the Euryarchaeota phylum showed the most activity on the rumen epithelium of low RFI (81.3 +/- 1.9%) and high RFI (76.4 +/- 3.0%) steers. Bacteria from the Succinivibrionaceae family showed the greatest activity of bacteria on the low RFI (28.7 +/- 9.0%) and high RFI (33.9 +/- 8.8%) epithelium. Of the bacterial families, Campylobacteraceae and Neisseriaceae had significantly greater activity on the low RFI epithelium (p < 0.05) and are known to play a role in oxygen scavenging. Greater activity of rumen epithelial attached oxygen scavenging bacteria may provide more optimal feed fermentation conditions, which contributes to high fermentation efficiency in the rumen.

Automated summary

This study aimed to characterize the role of ruminal epithelial attached microbiota in cattle feed efficiency, identifying differences in transcriptionally active bacteria and archaea between cattle with low and high feed efficiency. The results showed that bacteria from the Succinivibrionaceae family and archaea from unclassified genera belonging to the Euryarchaeota phylum were more active in cattle with low feed efficiency, suggesting a potential impact on optimal feed fermentation conditions and high fermentation efficiency in the rumen.