Journal
CHEMICAL SCIENCE
Volume 12, Issue 47, Pages 15572-15580Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1sc05351e
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Funding
- Cancer Prevention Research Institute of Texas (CPRIT) [RR170014]
- NIH [R35GM133706, R21-CA255894]
- U.S. Department of Defense [W81XWH-21-1-0789]
- Robert A. Welch Foundation [C-1970, C-0807]
- National Science Foundation [CHE-1803066]
- Hamill Innovation Award (Hamill Foundation)
- John S. Dunn Foundation Collaborative Research Award (Gulf Coast Consortia)
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In this study, a series of photoactivatable probes were prepared using the oxime moiety as a new class of photolabile caging group. The oxime-caged fluorophores can be oxidized to their carbonyl derivatives upon light irradiation, restoring strong fluorophore fluorescence. Oximes represent a new class of visible-light photocages that can be widely used for cellular imaging, sensing, and photo-controlled molecular release.
Photoactivatable fluorophores have been widely used for tracking molecular and cellular dynamics with subdiffraction resolution. In this work, we have prepared a series of photoactivatable probes using the oxime moiety as a new class of photolabile caging group in which the photoactivation process is mediated by a highly efficient photodeoximation reaction. Incorporation of the oxime caging group into fluorophores results in loss of fluorescence. Upon light irradiation in the presence of air, the oxime-caged fluorophores are oxidized to their carbonyl derivatives, restoring strong fluorophore fluorescence. To demonstrate the utility of these oxime-caged fluorophores, we have created probes that target different organelles for live-cell confocal imaging. We also carried out photoactivated localization microscopy (PALM) imaging under physiological conditions using low-power light activation in the absence of cytotoxic additives. Our studies show that oximes represent a new class of visible-light photocages that can be widely used for cellular imaging, sensing, and photo-controlled molecular release.
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