Journal
VETERINARY AND ANIMAL SCIENCE
Volume 14, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.vas.2021.100217
Keywords
E. coli; Probiotic; Cephalosporin resistance; Pig; Microbiota
Funding
- French Ministry of Agriculture, Agro-Food and Forestry [2017-389]
- Cotes d'Armor Departmental Council
- Fondation pour la Recherche Medicale (Equipe FRM 2016) [DEQ20161136698]
- ANSES (the French Agency for Food, Environmental and Occupational Health Safety)
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The study evaluated the impact of administrating two Escherichia coli probiotic strains on the gut carriage or shedding of extended-spectrum beta-lactamase-producing E. coli in pigs, finding that the probiotics did not reduce the shedding of the challenge strain.
We evaluated the impact of the administration of two Escherichia coli probiotic strains (ED1a and Nissle 1917) to pigs on the gut carriage or shedding of extended-spectrum beta-lactamase-producing E. coli. The probiotics were given to four sows from 12 days before farrowing to the weaning day, and to the 23 piglets (infected treated group (IPro)) from birth to the age of 49 days. Four other sows and their 24 piglets (infected non-treated group (INT)) did not receive the probiotics. IPro and INT piglets (n = 47) were orally inoculated with the strain E. coli 17-348F-RifR carrying the bla(CTX-M-1) gene and resistant to rifampicin. Cefotaxime-resistant (CTXR) E. coli and rifampicin-resistant (Rif(R)) E. coli were cultured and excretion of probiotics was studied using PCR on individual faecal and post-mortem samples, and from manure collected after the challenge with resistant E. coli. CTXR and Rif(R) E. coli isolates were characterized to detect transfer of the bla(CTX-M-1) to other strains.. Overall, there was no significant reduction in faecal excretion of CTXR and Rif(R) E. coli in IPro pigs compared with INT pigs, although the CTXR and Rif(R) E. coli titres were slightly, but significantly lower in the colon, caecum and rectum at post mortem. Excretion of the probiotics decreased with age, but Nissle 1917 was detected in most pigs at postmortem. No transfer of the bla(CTX-M-1) gene to probiotic and other E. coli strains was detected. In conclusion, in our experimental conditions, the used probiotics did not reduce shedding of the challenge strain.
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