Journal
ANALYTICAL METHODS
Volume 13, Issue 48, Pages 5813-5820Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1ay01460a
Keywords
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Funding
- National Key R&D Program of China [2018YFC1901000]
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A dual-channel assay was developed using gold nanoparticles and copper nanoclusters for colorimetric and fluorescent detection of kanamycin. The assay showed a low limit of detection for kanamycin and offered great opportunities for on-site monitoring of antibiotics in environmental samples.
In this work, a dual-channel assay was constructed for the colorimetric and fluorescent detection of kanamycin (KAN) based on gold nanoparticles (Au NPs) and copper nanoclusters (Cu NCs). Initially, the fluorescence of Cu NCs was quenched by 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (AHMT)-functionalized Au NPs due to the inner filter effect (IFE). The existence of KAN acted as a molecular bridge to interact with AHMT via hydrogen bonds and induced the aggregation of AHMT-Au NPs, leading to a change in the color of the gold colloidal solution from reddish-violet to blue within 2 min. Moreover, the aggregated AHMT-Au NPs can weaken its IFE toward Cu NCs and result in fluorescence restoration. With the sensor employed here, the concentration of KAN can be quantitatively analyzed through double channels, and a low LOD (limit of detection) of 1.9 nM and 1.2 nM was realized by the colorimetric and fluorescent method, respectively. Benefitting from the short response time, high sensitivity, and good reliability, the established assay offered great opportunities for the on-site monitoring of antibiotics in environmental samples.
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