4.5 Article

A highly sensitive carbapenemase assay using laser desorption/ionization mass spectrometry based on a parylene-matrix chip

Journal

ENZYME AND MICROBIAL TECHNOLOGY
Volume 104, Issue -, Pages 56-68

Publisher

ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2017.05.010

Keywords

Antibiotic-susceptibility test; Carbapenem; Laser desorption/ionization mass spectrometry (LDI MS); MALDI-TOF MS; Parylene-matrix chip

Funding

  1. Nano-Convergence Foundation - Ministry of Science, ICT and Future Planning (MSIP, Korea) [R201602210]
  2. Industry Technology Development Program - Ministry of Trade, Industry and Energy (MOTIE, Korea), [10063335]
  3. National Research Foundation of Korea [NRF-2017R1A2B4004077, NRF-2017R1A2B2004398]
  4. National Research Foundation of Korea [R201602210] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

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A quantitative carbapenemase assay was developed using laser desorption/ionization mass spectrometry (LDI-MS) based on a parylene-matrix chip. As a first step, the reproducibility (spot-to-spot, shot-to-shot,and day-to-day) of LDI-MS based on a parylene-matrix chip and the quantification ranges for four carbapenem antibiotics (doripenem, ertapenem, imipenem, and meropenem) were determined. A carbapenem-susceptibility test was performed using the four carbapenems and 51 bacterial strains that displayed (1) carbapenem resistance with carbapenemase, (2) carbapenem resistance without carbapenemase, or (3) carbapenem susceptibility. The susceptibility test results showed that LDI-MS based on a parylene-matrix chip was more sensitive and selective for detecting the carbapenemase reaction than conventional MALDI-TOF MS based on a 2,5-dihydroxybenzoic acid matrix.

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