Journal
ENZYME AND MICROBIAL TECHNOLOGY
Volume 106, Issue -, Pages 83-87Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2017.07.007
Keywords
Trichoderma reesei; Glucose oxidase; Secretion pathway; snc1; bip1; hac1
Categories
Funding
- National High Technology Research and Development Program of China [2013AA102803]
- National Natural Science Foundation of China [31400067, 31672458]
- National Key Research and Development Program of China [2016YED0501409-02]
- Special Fund for Agro-Scientific Research in the Public Interest [201403047]
- Elite Youth Program of Chinese Academy of Agricultural Sciences
Ask authors/readers for more resources
Vast interest exists in developing T. reesei for production of heterologous proteins. Although rich genomic and transcriptomic information has been uncovered for the T. reesei secretion pathway, little is known about whether engineering its key components could enhance expression of a heterologous gene. In this study, snc1, a v-SNARE gene, was first selected for overexpression in T. reesei. In engineered T. reesei with additional copies of snc1, the Aspergillus niger glucose oxidase (AnGOD) was produced to a significantly higher level(2.2-fold of the parental strain). hac1 and bip1, two more component genes in the secretion pathway, were further tested for over expression and found to be also beneficial for AnGOD secretion. The overexpression of one component gene more or less affected the expression of the other two genes, suggesting a complex regulating mechanism. Our study demonstrates the potential of engineering the secretion pathway for enhancing heterologous gene production in T. reesei.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available