4.6 Article

Kaempferol, a phytoestrogen, suppressed triclosan-induced poepithelial-mesenchymal transition and metastatic-related behaviors of MCF-7 breast cancer cells

Journal

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume 49, Issue -, Pages 48-57

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.etap.2016.11.016

Keywords

Kaempferol; Triclosan; Epithelial-mesenchymal transition; Metastasis; Breast cancer cells

Funding

  1. National Research Foundation of Korea (NRF) grant - Ministry of Education, Science and Technology (MEST) of the Republic of Korea [2014R1A1A2055295]
  2. Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ011355-2015]
  3. National Research Foundation of Korea [2014R1A1A2055295] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

Ask authors/readers for more resources

As a phytoestrogen, kaempferol is known to play a chemopreventive role inhibiting carcinogenesis and cancer progression. In this study, the influences of triclosan, an anti-bacterial agent recently known for an endocrine disrupting chemical (EDC), and kaempferol on breast cancer progression were examined by measuring their effects on epithelial-mesenchymal transition (EMT) and metastatic-related behaviors of MCF-7 breast cancer cells. Morphological changes of MCF-7 cells were observed, and a wound healing assay was performed after the treatment of triclosan and kaempferol. The effects of triclosan and kaempferol on protein expression of EMT-related markers such as E-cadherin, N-cadherin, Snail, and Slug and metastasis-related markers such as cathepsin B, D, MMP-2 and 9 were investigated by Western blot assay. In microscopic observations, triclosan (10(-6) M) or E2 (10(-9) M) induced transition to mesenchymal phenotype of MCF-7 cells compared with the control. Co-treatment of ICI 182,780 (10(-8) M), an ER antagonist, or kaempferol (25 mu M) with E2 or triclosan restored the cellular morphology to an epithelial phenotype. In a wound-healing scratch and a transwell migration assay, triclosan enhanced migration and invasion of MCF-7 cells, but co-treatment of kaempferol or ICI 182,780 reduced the migration and invasion ability of MCF-7 cells to the control level. In addition, kaempferol effectively suppressed E2 or triclosan-induced protein expressions of EMT and metastasis promoting markers. Taken together, triclosan may be a distinct xenoestrogenic EDC to promote EMT, migration, and invasion of MCF-7 breast cancer cells through ER. On the other hand, kaempferol can be an alternative chemopreventive agent to effectively suppress the metastatic behavior of breast cancer induced by an endogenous estrogen as well as exogenous xenoestrogenic compounds including triclosan. (C) 2016 Elsevier B.V. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available