Cryopreservation of Hazelnut (Corylus avellana L.) Axillary Buds from In Vitro Shoots Using the Droplet Vitrification Method

Review Biotechnology & Applied Microbiology

Advances in cryopreservation of in vitro-derived propagules: technologies and explant sources

Min-Rui Wang et al.

Summary: New technologies in in-vitro based cryopreservation systems have advanced the field of plant cryopreservation since the twenty first century, allowing for more diverse germplasm to be successfully preserved in cryobanks in the future.

PLANT CELL TISSUE AND ORGAN CULTURE (2021)

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Review Biotechnology & Applied Microbiology

Challenges in implementing plant shoot tip cryopreservation technologies

Jean Carlos Bettoni et al.

Summary: Successful implementation of plant shoot tip cryopreservation projects requires clear goals, long-term funding, skilled technical support staff, and necessary infrastructure. Cryopreservation is a safe approach for long-term preservation, but challenges such as technology transfer and protocol validation must be addressed. Multiple factors including plant material quality, method selection, and personnel training contribute to the success of cryopreservation projects.

PLANT CELL TISSUE AND ORGAN CULTURE (2021)

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Review Agriculture, Multidisciplinary

Advances in cultivar choice, hazelnut orchard management, and nut storage to enhance product quality and safety: an overview

Cristian Silvestri et al.

Summary: The main objective of the study is to systematize published information regarding recent findings about cultural operations that directly influence nut and kernel quality, support varietal choice for new plantations, and list recent advances in nut storage and quality and safety evaluation.

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2021)

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Review Chemistry, Multidisciplinary

Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges

Tie Chang et al.

Summary: Cryopreservation technology is crucial in biomedical applications, but the formation, growth, and recrystallization of ice crystals pose major challenges. Anti-icing materials and strategies can effectively reduce ice damage and enhance efficiency, including chemical ice-inhibition molecules, advanced engineering strategies, and external physical field technologies. Addressing current challenges and future perspectives in ice inhibition is essential for high-efficiency cryopreservation.

ADVANCED SCIENCE (2021)

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Review Plant Sciences

Cryopreservation of Woody Crops: The Avocado Case

Chris O'Brien et al.

Summary: The recent development and implementation of crop cryopreservation protocols have enhanced the ability to maintain recalcitrant seed germplasm collections via cryopreserved in vitro material. To preserve global plant genetic resources for future food security and breeding programs, integrating in situ and ex situ conservation methods into a cohesive conservation plan is essential, with tissue culture and cryopreservation techniques offering promising complementary tools. These techniques can be used to conserve crops difficult to maintain in seed banks, such as woody perennial plants, recalcitrant seed crops, and vegetatively propagated crops.

PLANTS-BASEL (2021)

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Review Biochemistry & Molecular Biology

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Cesar Augusto Roque-Borda et al.

Summary: Various environmental and endogenous factors influence genetic diversity in natural populations. Cryostorage plays a crucial role in preserving plant species and enhancing cold tolerance. Cryopreservation techniques, cryoprotective agents, and genetic integrity in plant cryobiology are essential considerations for successful preservation efforts.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

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Article Biodiversity Conservation