Journal
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
Volume 53, Issue -, Pages 145-150Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.etap.2017.05.013
Keywords
Dibutyl phthalate; Sertoli cells; Apoptosis
Funding
- Science and Technological Project of Jilin Province in China [20130101141JC]
- Nature and Science Fund from Jilin Province Ministry of Education [2016071]
- Health Technological Innovation Project of Jilin Province [2016J086]
- Young Teacher Enhancement Program
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Dibutyl phthalate (DBP) has significant male reproductive toxicity, and the Sertoli cells are the target cells of DBP. This study was to investigate the injury effect induced by DBP on rat testicular Sertoli cells in vitro. MTT results showed that DBP can significantly reduce the survival rate of Sertoli cells; Hoechst staining results showed that the Sertoli cells treated with DBP emerged with typical morphological characteristics of apoptosis, nuclear condensation and chromatin condensation; flow cytometry results showed that DBP significantly increased the apoptotic rate of Sertoli cells, and dose-dependent; Western blotting showed that the expression of PTEN protein in Sertoli cells was significantly higher than that in the control group after treated with different concentrations of DBP for 24 h, while the expression of p-PI3K1, p70S6K and 4E-BP1 protein in the PI3K/AKT/mTOR signal pathway were significantly decreased. It is speculated that PTEN/PI3K/AKT/mTOR signaling pathway plays an important role in DBP-induced apoptosis of testicular Sertoli cells in rats.
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