Journal
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Volume 62, Issue 14, Pages -Publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.62.14.23
Keywords
dynein-1; dynein heavy chain; conditional knockout
Categories
Funding
- NIH [EY08123, EY019298, EY014800039003, 5T32 EY024234]
- Research to Prevent Blindness (RPB
- New York)
- Retina Research Foundation-Houston (Alice McPherson, MD)
- RPB Senior Investigator award
- RPB Nelson Trust award
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This study demonstrates the essential role of dynein in photoreceptor development and maintenance in the retina, with the loss of dynein leading to rapid degeneration of photoreceptors. Dynein is crucial for the secretory pathway, outer segment formation, and photoreceptor maintenance.
PURPOSE. Cytoplasmic dynein-1 (henceforth dynein) moves cargo in conjunction with dynactin toward the minus end of microtubules. The dynein heavy chain, DYNC1H1, comprises the backbone of dynein, a retrograde motor. Deletion of Dync1h1 abrogates dynein function. The purpose of this communication is to demonstrate effects of photoreceptor dynein inactivation during late postnatal development and in adult retina. METHODS. We mated Dync1h1(F/F) mice with iCre75 and Prom1-CreER(T2) mice to generate conditional rod and tamoxifen-induced knockout in rods and cones, respectively. We documented retina degeneration with confocal microscopy at postnatal day (P) 10 to P30 for the iCre75 line and 1 to 4 weeks post tamoxifen induction (wPTI) for the Prom1-CreER(T2) line. We performed scotopic and photopic electroretinography (ERG) at P16 to P30 in the iCre75 line and at 1-week increments in the Prom1-CreER(T2) line. Results were evaluated statistically using Student's t-test, two-factor ANOVA, and Welch's ANOVA. RESULTS. Cre-induced homologous recombination of Dync1h1(F/F) mice truncated DYNC1H1 after exon 23. (rod)Dync1h1(-/-) photoreceptors degenerated after P14, reducing outer nuclear layer (ONL) thickness and combined inner segment/outer segment (IS/OS) length significantly by P18. Scotopic ERG a-wave amplitudes decreased by P16 and were extinguished at P30. Cones were stable under rod-knockout conditions until P21 but inactive at P30. In (tam)Dync1h1(-/-) photoreceptors, the IS/OS began shortening by 3wPTI and were nearly eliminated by 4wPTI. The ONL shrank significantly over this interval, indicating rapid photoreceptor degeneration following the loss of dynein. CONCLUSIONS. Our results demonstrate dynein is essential for the secretory pathway, formation of outer segments, and photoreceptor maintenance.
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