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STAR PROTOCOLS
Volume 2, Issue 4, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2021.100900
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Deep immune profiling is crucial for understanding the human immune system, and successful biological interpretation relies on consistent laboratory processing and minimal batch-to-batch variation.
Deep immune profiling is essential for understanding the human immune system in health and disease. Successful biological interpretation of this data requires consistent laboratory processing with minimal batch-to-batch variation. Here, we detail a robust pipeline for the profiling of human peripheral blood mononu-clear cells by both high-dimensional flow cytometry and single-cell RNA-seq. These protocols reduce batch effects, generate reproducible data, and increase throughput. For complete details on the use and execution of this protocol, please refer to Savage et al. (2021).
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