Journal
STAR PROTOCOLS
Volume 2, Issue 4, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2021.101008
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Funding
- Royal Society [UF120046, RG080398]
- Moorfields Eye Charity [E170004A, R150032A, R180005A]
- Medical Research Council UK [mr/j004553/1, MR/T002735/1]
- Royal Society [UF120046] Funding Source: Royal Society
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This protocol outlines tracking retinal cell motility in live explanted mouse retinae, applicable to any fluorescently labeled cell, with careful tissue handling and further guidance for semi-automated data processing. For detailed usage and execution, refer to Aghaizu et al. (2021).
The developing retina undergoes dynamic organizational changes involving significant intra-retinal motility of the encompassing cells. Here, we present a protocol for tracking retinal cell motility in live explanted mouse retinae. Although originally applied to rod and cone photoreceptors, this strategy is applicable to any fluorescently labeled cell in mouse retinae and other similar experimental retinal models. Careful tissue handling is critical for the successful acquisition of high-quality live imaging data. Further instructions for semi-automated in silico data handling are provided.For complete details on the use and execution of this protocol, please refer to Aghaizu et al. (2021).
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