Impact of Surface Charge on Cerium Oxide Nanoparticle Uptake and Translocation by Wheat (Triticum aestivum)

Nanoparticle (NP), physiochemical properties, including surface charge, affect Cellular uptake, translocation, and tissue localization. To, evaluate the influence of surface charge on NP uptake by plants, wheat seedlings were hydroponically exposed to 20, mg/L of similar to 4nm CeO2 NPs funCtiOnalized with positively charged, negatively,charged, and neutral deitran coatings. Fresh, hydrated loots and leaves were analyzed at various time points over 34 h using fluorescence X-ray absorption near-edge spectroscOpy to provide-laterally resolved spatial-distribution and speciation of ce. A-,15:20% reduction-from Ce(IV) to Ce(III) was observed in both roots and leaves, independent of NP surface charge'. Because of its higher affinity with negatively charged cell walls, CeO2(+) NP's adhered to the plant roots the:strongest. After: 34 h, CeO2(-), and CeO2(0) NP exposed plants had higher Ce leaf concentrations than the plants exposed 'to CeO2(+) NPs. whereas Ce was fOund mostly in the leaf veins of the CeO2(-) NP exposed plant, 'Ca waS' found in clusters in the nonvascular leaf tissue of the CeO2(0) NP expoSed plant. These results provide important information for understanding mechanisms responsible for plant uptake, transformation, and translocation of NP's, and suggest'that-NP coatings can be designed to target NPs to specific parts of plants. [GRAPHICS]