Journal
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
Volume 19, Issue -, Pages 6315-6327Publisher
ELSEVIER
DOI: 10.1016/j.csbj.2021.11.030
Keywords
M-MuLV RT; Reverse transcriptase; Site-directed mutagenesis; Random mutagenesis; Fusion proteins
Funding
- Russian State-funded budget project of ICBFM SB RAS [121031300056-8]
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Reverse transcriptases (RTs) are crucial enzymes used in modern biotechnology and molecular diagnostics, with M-MuLV RT being the most commonly used one. Improvements in M-MuLV RT can enhance its practical utility for a wide range of applications.
Reverse transcriptases (RTs) are enzymes synthesizing DNA using RNA as the template and serving as the standard tools in modern biotechnology and molecular diagnostics. To date, the most commonly used reverse transcriptase is the enzyme from Moloney murine leukemia virus, M-MuLV RT. Since its discovery, M-MuLV RT has become indispensable for modern RNA studies; the range of M-MuLV RT applications is vast, from scientific tasks to clinical testing of human pathogens. This review will give a brief description of the structure, thermal stability, processivity, and fidelity, focusing on improving M-MuLV RT for practical usage. (C) 2021 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.
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