Spatial distribution of sediment archaeal and bacterial communities relates to the source of organic matter and hypoxia - a biogeographical study on Lake Remoray (France)

Bottom waters hypoxia spreads in many lakes worldwide causing severe consequences on whole lakes trophic network. Here, we aimed at understanding the origin of organic matter stored in the sediment compartment and the related diversity of sediment microbial communities in a lake with deoxygenated deep water layers. We used a geostatistical approach to map and compare both the variation of organic matter and microbial communities in sediment. Spatialisation of C/N ratio and delta 13C signature of sediment organic matter suggested that Lake Remoray was characterized by an algal overproduction which could be related to an excess of nutrient due to the close lake-watershed connectivity. Three spatial patterns were observed for sediment microbial communities after the hypoxic event, each characterized by specific genetic structure, microbial diversity and composition. The relative abundance variation of dominant microbial groups across Lake Remoray such as Cyanobacteria, Gammaproteobacteria, Deltaproteobacteria and Chloroflexi provided us important information on the lake areas where hypoxia occurs. The presence of methanogenic species in the deeper part of the lake suggests important methane production during hypoxia period. Taken together, our results provide an extensive picture of microbial communities' distribution related to quantity and quality of organic matter in a seasonally hypoxic lake.

Automated summary

Bottom waters hypoxia in lakes can have severe consequences on lake trophic networks. In Lake Remoray, excess algal production was linked to nutrient runoff from the watershed. After a hypoxic event, sediment microbial communities exhibited distinct spatial patterns and variations in abundance of dominant microbial groups, providing valuable insights into areas affected by hypoxia. Additionally, the presence of methanogenic species in deep lake areas suggests significant methane production during hypoxic periods. Overall, this study offers a comprehensive understanding of microbial community distribution in relation to organic matter in a seasonally hypoxic lake.