Journal
FOOD TECHNOLOGY AND BIOTECHNOLOGY
Volume 59, Issue 4, Pages 475-495Publisher
FACULTY FOOD TECHNOLOGY BIOTECHNOLOGY
DOI: 10.17113/ftb.59.04.21.7205
Keywords
human milk fat substitute; interesterification; lipase; sn-2 palmitate
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Human milk fat substitute (HMFS) is a structured lipid designed to resemble human milk fat, synthesized by the enzymatic interesterification of vegetable oils, animal fats or a blend of oils. The focus of the review is on the synthesis of HMFS by lipase-catalyzed interesterification, providing a detailed overview of biocatalysts, substrates, synthesis methods, and factors influencing the synthesis and purification process.
Human milk fat substitute (HMFS) is a structured lipid designed to resemble human milk fat. It contains 60-70 % palmitic acid at the sn-2 position and unsaturated fatty acids at the sn-1, 3 positions in triacylglycerol structures. HMFS is synthesized by the enzymatic interesterification of vegetable oils, animal fats or a blend of oils. The efficiency of HMFS synthesis can be enhanced through the selection of appropriate substrates, enzymes and reaction methods. This review focuses on the synthesis of HMFS by lipase-catalyzed interesterification and provides a detailed overview of biocatalysts, substrates, synthesis methods, factors influencing the synthesis and purification process for HMFS production. Major challenges and future research in the synthesis of HMFS are also discussed. This review can be used as an information for developing future strategies in producing HMFS.
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