A Simple and Sensitive HPLC-UV Method for the Estimation of Ofloxacin in Plasma

The objective of the study was to develop an HPLC-UV method for the analysis of ofloxacin in plasma. An HPLC-UV method has been developed using C18 column with UV detection at 294 nm. The expected optimal assay condition comprised of methanol and phosphate buffer pH 7.4 in ratio of 40:60 % v/v at a flow rate of 1 mL/min. Under this optimal state, detection of ofloxacin with good resolution and retention time less than 5 min were attained. The method was proved to be linear in the range of 100 to 600 ng/mL and 0.9997 regression values was obtained for ofloxacin. The percentage recovery of ofloxacin was ranged from 96.29 to 99.57 %. The precision and selectivity of the developed method was good and can be used for the estimation of ofloxacin in plasma. The developed method can be applied for the estimation of pharmacokinetics of ofloxacin.

Automated summary

The study developed an HPLC-UV method for analyzing ofloxacin in plasma, showing good linearity, recovery rate, and resolution, which can be used for estimating the pharmacokinetics of ofloxacin.