Journal
GLYCOBIOLOGY
Volume 31, Issue 11, Pages 1571-1581Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwab086
Keywords
chondroitin sulfate; chondroitinase ABC I; crystal structure; kinetic analysis; substrate specificity
Categories
Ask authors/readers for more resources
cABC-I exhibits the highest reactivity to CSA and CSC among all CS isomers, with a higher kcat/Km for CSA. The crystal structures revealed the interaction between Arg500 and 4-O-sulfated and 6-O-sulfated GalNAc residues, suggesting a higher affinity for CSA due to the closer distance between Arg500 and the 4-O-sulfate group. These findings provide insight into the pharmacological action of cABC-I in treating lumbar disc herniation.
Chondroitinase ABC I (cABC-I) is the enzyme which cleaves the beta-1,4 glycosidic linkage of chondroitin sulfate (CS) by beta-elimination. To elucidate more accurately the substrate specificity of cABC-I, we evaluated the kinetic parameters of cABC-I and its reactivity with CS isomers displaying less structural heterogeneity as substrates, e.g., approximately 90 percent of disaccharide units in Chondroitin sulfate A (CSA) or Chondroitin sulfate C (CSC) is D-glucuronic acid and 4-Osulfated N-acetyl galactosamine (GalNAc) (A-unit) or D-glucuronic acid and 6-O-sulfated GalNAc (C-unit), respectively. cABC-I showed the highest reactivity to CSA and CSC among all CS isomers, and the kcat/Km of cABC-I was higher for CSA than for CSC. Next, we determined the crystal structures of cABC-I in complex with CS disaccharides, and analyzed the crystallographic data in combination with molecular docking data. Arg500 interacts with 4-O-sulfated and 6-O-sulfated GalNAc residues. The distance between Arg500 and the 4-O-sulfate group was 0.8 A shorter than that between Arg500 and the 6-O-sulfated group. Moreover, it is likely that the 6-O-sulfated group is electrostatically repulsed by the nearby Asp490. Thus, we demonstrated that cABC-I has the highest affinity for the CSA richest in 4-O-sulfated GalNAc residues among all CS isomers. Recently, cABC-I was used to treat lumbar disc herniation. The results provide useful information to understand the mechanism of the pharmacological action of cABC-I.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available