Journal
MACROHETEROCYCLES
Volume 14, Issue 2, Pages 153-156Publisher
IVANOVO STATE UNIV CHEMICAL TECHNOLOGY
DOI: 10.6060/mhc201128d
Keywords
Cobalamins; hypothiocyanite; lactoperoxidase; thioeyanate; coordination
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Funding
- Russian Science Foundation [19-73-00147]
- Russian Science Foundation [19-73-00147] Funding Source: Russian Science Foundation
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The study investigated the reactions between cobalamins and hypothiocyanite (OSCN-), showing that different types of cobalamins react differently in the presence of OSCN-. The formation of hypothiocyanitocobalamin is influenced by various factors, such as the type of cobalamin and the presence of other ligands.
Here, we report the results of investigation of the reactions between cobalamins (Cbls) and hypothiocyanite (OSCN-), a product of thiocyanate oxidation by hydrogen peroxide in the presence of lactoperoxidase. In the case of aquacobalamin, the product, the UV-vis spectrum of which differs from the UV-vis spectra of Cbl(III)-complexes with SCN-, SO32-, OCN- and CN- anions, is formed and attributed to hypothiocyanitocobalamin. This complex reacts with selenomethionine at substantially lower rate than the free OSCN-. Cyano- and methyl-Cbls are relatively stable in the presence of OSCN-. Glutathionylcobalamin is transformed to hypothiocyanitocobalamin in the presence of OSCN- excess via the oxidation of glutathionyl-ligand and subsequent binding of OSCN- by Cbl(III).
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