Journal
FEMS MICROBIOLOGY LETTERS
Volume 368, Issue 20, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/femsle/fnab141
Keywords
HtrA; L. monocytogenes; extracellular matrix interaction; proteolysis; ECM degradation; host proteins
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Funding
- Science and Engineering Research Board (SERB), Government of India [CRG/2019/003342]
- Austrian Science Fund (FWF) [I_4360]
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HtrA is a secreted virulence factor in many pathogenic bacteria, with varying interactions with ECM molecules and host proteins. The PDZ domain plays a crucial role in substrate cleavage, potentially contributing to the pathogenicity of HtrA in L. monocytogenes.
High Temperature Requirement A (HtrA) was identified as a secreted virulence factor in many pathogenic bacteria, including Listeria monocytogenes. Recently, it was discovered that Helicobacter pylori and Campylobacter jejuni HtrAs can directly cleave the human cell-adhesion molecule E-cadherin, which facilitates bacterial transmigration. HtrAs also interact with extracellular matrix (ECM) molecules. However, only a limited number of studies have been carried out in this regard. In the present study, the protease and ECM binding properties of L. monocytogenes HtrA (LmHtrA) were studied using native rLmHtrA, catalytically inactive rLmHtrA(S343A) and rLmHtrA lacking the PDZ domain (Delta PDZ) to gain more insights into HtrA-ECM molecule interaction. The results show that (1) native rLmHtrA cleaves fibrinogen, fibronectin, plasminogen and casein in a time and temperature dependent manner, (2) interaction of rLmHtrA with various host proteins was found in the micromolar to nanomolar range, (3) in the absence of PDZ domain, rLmHtrA exhibits no drastic change in binding affinity toward the host molecules when compared with native rLmHtrA and (4) the PDZ domain plays an important role in the substrate cleavage as rLmHtrA(1-394)Delta PDZ cleaves the substrates only under certain conditions. The proteolysis of various ECM molecules by rLmHtrA possibly highlights the role of HtrA in L. monocytogenes pathogenesis involving ECM degradation.
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