Journal
CROP JOURNAL
Volume 9, Issue 6, Pages 1357-1366Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.cj.2021.02.012
Keywords
Aegilops tauschii; Triticum tauschii; Lr42; KASP; Diagnostic marker
Categories
Funding
- Kansas Agricultural Experiment Station [21-071-J]
- National Research Initiative Competitive from US Department of Agriculture [2017-67007-25939]
- National Institute of Food and Agriculture
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The study identified diagnostic DNA markers for Lr42 by utilizing sequence polymorphisms of a differentially expressed gene TaRPM1. These markers show superior selection accuracy compared to previously used markers, with the advantages of low cost and easy assay, suitable for marker-assisted selection in breeding programs.
Wheat leaf rust is a prevalent foliar disease in wheat worldwide. Growing resistant cultivars is an effective strategy to minimize the impact of leaf rust on yield and grain quality. Lr42 is a leaf rust resistance gene identified from Aegilops tauschii and is still effective against current predominant leaf rust races in the United States and many other countries. In this study, we developed diagnostic DNA markers for Lr42 using the sequence polymorphisms of a differentially expressed gene (TaRPM1) encoding a putative NBARC protein in the Lr42 candidate region identified by RNA-sequencing of two near-isogenic lines contrasting in Lr42 alleles. Markers were designed based on a deletion mutation and a single nucleotide polymorphism (SNP) in the gene. Haplotype analyses of the newly developed markers in the three diversity panels demonstrated that they are diagnostic for Lr42, and superior to previously used markers in selection accuracy. These markers have the advantages of low cost and easy assay, and they are suitable for marker-assisted selection in breeding programs with either high- or low-throughput marker screening facilities. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
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