Journal
2021 IEEE SENSORS
Volume -, Issue -, Pages -Publisher
IEEE
DOI: 10.1109/SENSORS47087.2021.9639797
Keywords
Paper-based sensor; MicroRNA analyzation; Exponential Amplification Reaction
Funding
- National Science Foundation [ECCS 16-53673]
- National Institute of Food and Agriculture [201867021-27968]
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This study presents a paper-based miRNA sensor system that can detect miRNA-223 with a concentration as low as 10 pM within one hour.
Short non-coding RNAs, which are known as microRNAs (miRNAs), play a key role in fundamental cellular processes by post-transcription regulations. This paper reports a paper-based miRNA exponential amplification and detection system for rapid analysis of miRNAs. The paper-based sensor system consists of a silicone frame, glass microfiber pads for the storage of reaction reagents, a polyethersulfone flow channel for sample delivery, and two transparent and adhesive films to seal the glass microfiber pads inside reaction chambers. The results showed that the paper-based miRNA sensor can detect miRNA-223 with a concentration as low as 10 pM within one hour.
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