Peptidomic analysis characterising proteolysis in thaw-aging of beef short plate

Recent studies have suggested that thaw-aging can improve sensory attributes of freeze-thawed meat. Acceleration of proteolysis is expected to promote tenderisation and improve taste; however, the details of protein degradation, including substrate proteins and cleavage sites, remain unclear. Here, we report a time course overview of the peptidome of beef short plates during thaw-aging. The accelerated degradation of key proteins for meat tenderisation, such as troponin T and desmin, was confirmed. Additionally, 11 cleavage sites in troponin T related to taste-active peptide generation were identified. Terminome analysis showed that the contribution of each protease varies depending on the substrate proteins and the thaw-aging period. Based on our results; proteases, not only calpains, but also others contributed to the degradation of myofibrillar proteins. The techniques employed indicate that meat proteolysis during thaw-aging is not constant but dynamic.

Automated summary

Thaw-aging improves sensory attributes of freeze-thawed meat by accelerating proteolysis, which promotes tenderisation and taste improvement. Key proteins like troponin T and desmin are degraded, with multiple cleavage sites identified, and different proteases contribute to myofibrillar protein degradation during thaw-aging, indicating a dynamic process.