Expression analysis of genes for cytochrome P450 CYP86 and glycerol-3-phosphate acyltransferase related to suberin biosynthesis in rice roots under stagnant deoxygenated conditions

The radial oxygen loss (ROL) barrier formed on the outer cell layers of roots of rice (Oryza sativa) contributes to efficient oxygen transfer through the aerenchyma from the aerial parts to the root apex. It is hypothesized that suberin accumulation in the exodermis contributes to an apoplastic barrier and plays a vital role in ROL barrier formation. A previous study reported that some genes encoding cytochrome P450 family 86 (CYP86) and glycerol-3-phosphate acyltransferase (GPAT) might be involved in suberin biosynthesis during ROL barrier development of rice roots. However, how these genes are expressed and their contribution to the sequential development of suberin accumulation in rice roots remains unclear. In this study, four CYP86 and five GPAT genes of rice were identified as candidate genes involved in suberin biosynthesis in roots using sequence homology alignment with Arabidopsis suberin and cutin biosynthesis genes. Gene expression analyses revealed that expression of the candidate genes was induced at the region where suberin biosynthesis occurred under stagnant deoxygenated conditions. These genes showed two types of spatiotemporal expression patterns, at the regions of 5-25 mm and 25-35 mm from the root apex. Tissue-specific expression analyses using laser microdissection and histochemical GUS staining revealed that candidate gene expression was similar in the cell layer of the root exodermis. These results suggest that the selected CYP86 and GPAT genes are involved in suberin biosynthesis in the exodermis, and that suberin biosynthesis in the root may be controlled by the spatiotemporal expression of two groups of genes.

Automated summary

The radial oxygen loss (ROL) barrier on the outer cell layers of rice roots helps efficient oxygen transfer, with genes CYP86 and GPAT identified as potentially involved in suberin biosynthesis. Under deoxygenated conditions, these genes were induced and showed spatiotemporal expression patterns near the root apex. This suggests that the selected genes are important for suberin biosynthesis in the root exodermis and may be controlled by spatiotemporal expression.