Journal
MOLECULES
Volume 26, Issue 20, Pages -Publisher
MDPI
DOI: 10.3390/molecules26206270
Keywords
cassava wastewater; fermentation; bioreactor; S. marcescens; prodigiosin; alpha-glucosidase inhibitor; anti-diabetes; docking study
Funding
- Prince of Songkla University
- Ministry of Higher Education, Science, Research and Innovation under the Reinventing University Project [REV64020]
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In this study, cassava wastewater was used for scaled-up production of prodigiosin (PG), which showed moderate antioxidant, high anti-NO, and potential alpha-glucosidase inhibitory activities. The results suggest that PG could serve as a potential antidiabetic drug candidate, with a better binding energy score compared to the commercial drug acarbose.
The purpose of this study was to reuse cassava wastewater (CW) for scaled-up production, via the fermentation of prodigiosin (PG), and to conduct an evaluation of its bioactivities. PG was produced at the yield of high 6150 mg/L in a 14 L-bioreactor system, when the designed novel medium (7 L), containing CW and supplemented with 0.25% casein, 0.05% MgSO4, and 0.1% K2HPO4, was fermented with Serratia marcescens TNU01 at 28 degrees C in 8 h. The PG produced and purified in this study was assayed for some medical effects and showed moderate antioxidant, high anti-NO (anti-nitric oxide), and potential alpha-glucosidase inhibitory activities. Notably, PG was first reported as a novel effective alpha-glucosidase inhibitor with a low IC50 value of 0.0183 mu g/mL. The commercial anti-diabetic drug acarbose was tested for comparison and had a lesser effect with a high IC50 value of 328.4 mu g/mL, respectively. In a docking study, the cation form of PG (cation-PG) was found to bind to the enzyme alpha-glucosidase by interacting with two prominent amino acids, ASP568 and PHE601, at the binding site on the target enzyme, creating six linkages and showing a better binding energy score (-14.6 kcal/mol) than acarbose (-10.5 kcal/mol). The results of this work suggest that cassava wastewater can serve as a low-cost raw material for the effective production of PG, a potential antidiabetic drug candidate.
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