Journal
PROGRESS IN BIOMEDICAL ENGINEERING
Volume 3, Issue 1, Pages -Publisher
IOP Publishing Ltd
DOI: 10.1088/2516-1091/abbf5e
Keywords
molecular diagnostics; point-of-care; CRISPR; precision medicine; personalized medicine; gene editing; nucleic acid detection
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada [RGPIN-04151-2018]
- Ontario Research Fund [ORF-RE09-016]
- National Institutes of Health (NIH) [R01 AI117064-01]
- Air Force Office of Scientific Research [AFOSR FA2386-13-1-4120]
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The rapid development of CRISPR-based molecular diagnostics enables fast, sensitive, affordable, and precise molecular detection, with great potential in various critical applications.
Simple yet powerful clustered regularly-interspaced short palindromic repeats (CRISPR) technology has led to the advent of numerous developments in life sciences, biotechnology, therapeutics, and molecular diagnostics, enabled by gene editing capability. By exploiting the CRISPR-Cas system's nucleic acid sequence detection abilities, CRISPR-based molecular diagnostics have been developed. Here, we review the development of rapid, sensitive, and inexpensive CRISPR-based molecular diagnostics. We introduce the transition of CRISPR technology to precision molecular diagnostic devices from tube to device. Next, we discuss the various nucleic acid (NA) detection methods by CRISPR. We address the importance of significant sample preparation steps for a future sample-to-answer solution, which is lacking in current CRISPR-based molecular diagnostic technology. Lastly, we discuss the extension of CRISPR-based molecular diagnostics to various critical applications. We envision CRISPR technology holds great promise for widespread use in precision NA detection applications after particular technical challenges are overcome.
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