recount3: summaries and queries for large-scale RNA-seq expression and splicing

We present recount3, a resource consisting of over 750,000 publicly available human and mouse RNA sequencing (RNA-seq) samples uniformly processed by our new Monorail analysis pipeline. To facilitate access to the data, we provide the recount3 and snapcount R/Bioconductor packages as well as complementary web resources. Using these tools, data can be downloaded as study-level summaries or queried for specific exon-exon junctions, genes, samples, or other features. Monorail can be used to process local and/or private data, allowing results to be directly compared to any study in recount3. Taken together, our tools help biologists maximize the utility of publicly available RNA-seq data, especially to improve their understanding of newly collected data. recount3 is available from http://rna.recount.bio.

Automated summary

recount3 is a resource containing over 750,000 publicly available human and mouse RNA sequencing samples processed by the new Monorail analysis pipeline. Access to the data is facilitated through the recount3 and snapcount R/Bioconductor packages, along with complementary web resources. Monorail can process local and private data, allowing researchers to compare their results directly to any study in recount3.