Journal
JOURNAL OF ORTHOPAEDIC TRANSLATION
Volume 5, Issue -, Pages 16-25Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jot.2015.08.005
Keywords
cell migration; osseointegration; osteoconduction; titanium coatings; type I collagen
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Funding
- National Natural Science Foundation of China [51232007, 51172264, 31200718]
- Shanghai Science and Technology Development Fund [13DZ2294000]
- Opening Project of the Key Laboratory of Inorganic Coating Materials, Chinese Academy of Sciences [KLICM-2014-04]
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Background/Objective: Plasma-sprayed titanium coating (TC) with rough surfaces has been successfully applied in hip or knee prostheses. This study aimed to investigate the osteoconduction and osseointegration of Type I collagen covalently immobilised on TC (TC-AAC) compared with those of TC. Methods: In vitro, the migration of human mesenchymal stem cells (hMSCs) on TC and TC-AAC was observed by scanning electron microscopy and visualised fluorescent live/dead assay. In vivo, a rabbit model with femur condyle defect was employed, and implants of TC and TC-AAC were embedded into the femur condyles. Results: Collagen immobilised on TC could promote hMSCs' migration into the porous structure of the TC. Micro computed tomography images showed that bone trabeculae were significantly more abundant around TC-AAC implants than around TC implants. Fluorescence micrographs indicated more active new-bone formation around implants in the TC-AAC group than in the TC group. The measurement of boneeimplant contact on histological sections indicated significantly greater osteointegration around TC-AAC implants than around TC ones. Conclusion: Immobilised Type I collagen could improve the osteoconduction and osseointegration of TC implants. Copyright (C) 2015, The Authors. Published by Elsevier (Singapore) Pte Ltd.
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