Journal
FORENSIC SCIENCES RESEARCH
Volume 7, Issue 3, Pages 503-509Publisher
OXFORD UNIV PRESS
DOI: 10.1080/20961790.2020.1719454
Keywords
Forensic sciences; forensic pathology; vital reaction; skin burn; miR-711; miR-183-3p
Categories
Funding
- National Natural Science Foundation of China [81871526, 81601641]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars
- National Education Ministry [2015-311]
Ask authors/readers for more resources
In forensic practice, the identification of antemortem and postmortem burns is crucial. This study compared the expression of two miRNAs, miR-711 and miR-183-3p, in burned skins of mice and postmortem humans. The findings suggest that miR-711 and miR-183-3p may serve as biomarkers for vital reaction of skin burn.
In forensic practice, the identification of antemortem burns and postmortem burns is of the utmost importance. Reports from previous studies have shown that miRNAs, with lengths stretching over 18-25 nucleotides, are highly stable and resistant to degradation. However, there has been little research into the application of miRNAs in identifying antemortem and postmortem burns. This study compared the expression of miR-711 and miR-183-3p levels in mouse and postmortem human burned skins using RT-qPCR assay. RT-qPCR examination of burned mouse skins showed that increased miR-711 and miR-183-3p expression in comparison to intact skin tissues. The increased expressions of these two miRNAs were observed until 120 h after death in burned mouse skins, whereas no significant changes were found in postmortem burned skins. In human burned skins, the increased levels of these two miRNAs at 48 h following autopsy occurred in 19 of 26 subjects, which appeared to be related to the severity of the burn. These findings suggest that miR-711 and miR-183-3p may act as biomarkers for vital reaction of skin burn.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available