Journal
BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY
Volume 12, Issue 1, Pages 279-291Publisher
BIOINTERFACE RESEARCH APPLIED CHEMISTRY
DOI: 10.33263/BRIAC121.279291
Keywords
aldonic acids; maltobionic acid; sodium maltobionate; kinetic parameters; operational stability
Categories
Funding
- coordination of Improvement of Higher Education Personnel - Brazil (CAPES) [001]
- National Council for Scientific and Technological Development (CNPq)
- Research Support Foundation of the State of Rio Grande do Sul (FAPERGS)
- URI Erechim
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This study focused on immobilizing Z. mobilis cells containing GFOR/GL in flexible polyurethane foam to produce maltobionic acid. The maximum enzymatic activity was 19.40 U g(dry cell)(-1) with an immobilization yield of 60.29% and 15 reuses. The bioconversion process resulted in the production of 491.42 mmol L-1 of sodium maltobionate in 47.56 h, with high conversion factor and productivity rates.
Maltobionic acid and its salts are produced by the action of the periplasmic enzymatic complex glucose-fructose oxidoreductase (GFOR), and glicono-d-lactonase (GL) from Zymomonas mobilis and, for such, cell immobilization is outstanding. Thus, the objective of this work was to immobilize, in situ, Z. mobilis cells containing GFOR/GL in flexible polyurethane foam (PU) in order to produce maltobionic acid. In the immobilization, concentrations of polyurethane and biomass support constituents varied, and the visual aspect of the material immobilized was assessed, its enzymatic activity, immobilization yield, and operational stability. In the maximized immobilization condition, morphological analysis of the support and bioconversion runs were performed. The scanning electron microscopy analysis showed that the microorganism was trapped in the PU (7 g of polyol; 3.5 g of isocyanate; 0.02 g of silicone, and 7 g of biomass). The immobilized material maximum enzymatic activity against glucose was 19.40 U g(dry cell)(-1), with an immobilization yield of 60.29% and 15 reuses. In bioconversion, 491.42 mmol L-1 of sodium maltobionate were produced in 47.56 h, with a conversion factor of 0.88 and mass productivity, specific productivity, and specific product formation speed of 2.58 mmol h(-1); 1.81 mmol g(-1) h(-1) and 6.25 mmol g(-1) h(-1), respectively. This study's results contribute to the production of aldonic acids because it uses scarce information in the area and application potentials.
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