Functional characterization of C-TERMINALLY ENCODED PEPTIDE (CEP) family in Brassica rapa L

The small regulatory C-TERMINALLY ENCODED PEPTIDE (CEP) peptide family plays crucial roles in plant growth and stress response. However, little is known about this peptide family in Brassica species. Here, we performed a systematic analysis to identify the putative Brassica rapa L. CEP (BrCEP) gene family. In total, 27 BrCEP genes were identified and they were classified into four subgroups based on the CEP motifs similarity. BrCEP genes displayed distinct expression patterns in response to both developmental and several environmental signals, suggesting their broad roles during Brassica rapa development. Furthuremore, the synthetic BrCEP3 peptide accelerated Brassica rapa primary root growth in a hydrogen peroxide (H2O2) and Ca2+ dependent manner. In summary, our work will provide fundamental insights into the physiological function of CEP peptides during Brassica rapa development.

Automated summary

The C-terminally Encoded Peptide (CEP) gene family in Brassica species consists of 27 genes that are classified into four subgroups based on the similarity of CEP motifs. These genes play important roles in Brassica rapa development by responding to various environmental signals. Experimental results show that the synthetic BrCEP3 peptide can promote primary root growth in a hydrogen peroxide and Ca2+ dependent manner.