Journal
GELS
Volume 8, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/gels8020066
Keywords
nickel alginate; cobalt alginate; polyhistidine-tagged proteins; biopolymer; hydrogel
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This study presents a method of immobilizing proteins using alginate hydrogels, which can effectively prevent protein denaturation and have low cytotoxicity. The hydrogels can bind with polyhistidine-tagged proteins through interaction with crosslinking cations.
Protein immobilization using biopolymer scaffolds generally involves undesired protein loss of function due to denaturation, steric hindrance or improper orientation. Moreover, most methods for protein immobilization require expensive reagents and laborious procedures. This work presents the synthesis and proof of concept application of two alginate hydrogels that are able to bind proteins with polyhistidine tags by means of interaction with the crosslinking cations. Nickel (II) and cobalt (II) alginate hydrogels were prepared using a simple ionic gelation method. Hydrogels were characterized by optical microscopy and AFM, and evaluated for potential cytotoxicity. In addition, binding capacity was tested towards proteins with or without HisTAG. Hydrogels had moderate cytotoxicity and were able to exclusively bind polyhistidine-tagged proteins with a binding capacity of approximately 300 mu g EGFP (enhanced green fluorescent protein) per 1 mL of hydrogel. A lyophilized hydrogel-protein complex dissolved upon the addition of PBS and allowed the protein release and regain of biological activity. In conclusion, the nickel (II) and cobalt (II) alginate biopolymers provided an excellent platform for the carry and release of polyhistidine-tagged proteins.
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