Journal
SCIENCE ADVANCES
Volume 2, Issue 3, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.1501438
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Funding
- NSF [CHE-1058727, CHE-1363008, CHE1416268]
- NIH [1R21EB018014]
- Department of Defense Congressionally Directed Medical Research Programs Breast Cancer [W81XWH-12-1-0159/BC112431]
- Pratt School of Engineering Research Innovation Seed Fund Grant
- Duke University
- Burroughs Wellcome Fellowship
- American Chemical Society Petroleum Research Fund
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1416268, 1363008, GRANTS:13801355] Funding Source: National Science Foundation
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Conventional magnetic resonance (MR) faces serious sensitivity limitations which can be overcome by hyperpolarization methods, but the most common method (dynamic nuclear polarization) is complex and expensive, and applications are limited by short spin lifetimes (typically seconds) of biologically relevant molecules. We use a recently developed method, SABRE-SHEATH, to directly hyperpolarize N-15(2) magnetization and long-lived N-15(2) singlet spin order, with signal decay time constants of 5.8 and 23 min, respectively. We find >10,000-fold enhancements generating detectable nuclear MR signals that last for over an hour. N-15(2)-diazirines represent a class of particularly promising and versatile molecular tags, and can be incorporated into a wide range of biomolecules without significantly altering molecular function.
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