Journal
PNAS NEXUS
Volume 1, Issue 1, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pnasnexus/pgac028
Keywords
clinical samples; microfluidics; biosensor; smartphone; COVID-19; immunoassay; point-of-care; smartphone-based fluorescence microscope
Funding
- University of Arizona's Test All Test Smart program and Tech Launch Arizona's Asset Development program
- University of Arizona NASA Space Grant Graduate Fellowship
- Royal Thai Government Scholarship from the Ministry of Higher Education, Science, Research, and Innovation, Thailand
- Computational and Mathematical Modeling of Biomedical Systems Training Grant from the National Institute of General Medical Sciences (NIGMS)
- US National Institutes of Health [GM132008]
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A highly sensitive and low-cost method for diagnosing SARS-CoV-2 using clinical saline gargle samples has been developed using paper microfluidic chips and a smartphone-based fluorescence microscope.
Saliva specimens have drawn interest for diagnosing respiratory viral infections due to their ease of collection and decreased risk to healthcare providers. However, rapid and sensitive immunoassays have not yet been satisfactorily demonstrated for such specimens due to their viscosity and low viral loads. Using paper microfluidic chips and a smartphone-based fluorescence microscope, we developed a highly sensitive, low-cost immunofluorescence particulometric SARS-CoV-2 assay from clinical saline gargle samples. We demonstrated the limit of detection of 10 ag/mu L. With easy-to-collect saline gargle samples, our clinical sensitivity, specificity, and accuracy were 100%, 86%, and 93%, respectively, for n = 27 human subjects with n = 13 RT-qPCR positives.
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