EFFECT OF SHRNA INTERFERING B7-H3 GENE EXPRESSION ON THE PROLIFERATION AND INVASION OF BLADDER UROTHELIAL CARCINOMA T24 CELLS

Objective: To investigate the effect of shRNA interference on the proliferation and invasion of bladder urothelial carcinoma T24 cells. Methods: Thirty cases of bladder urothelial cell carcinoma and adjacent normal tissues were randomly selected from March 2019 to February 2020 in our hospital. The expression of B7-H3 in bladder urothelial carcinoma and adjacent normal tissues was detected by immunohistochemistry. T24 bladder cancer cells were cultured and infected with lentivirus. After culturing, B7-H3 low expression group and control group cells were obtained. Each group of cells was set with 4 multiple holes. The expression levels of B7-H3 and matrix metalloproteinase-2 (MMP-2) were measured via real-time fluorescent quantitative PCR and Western blotting. MTT assay methods were used to determine the proliferation of cells in each group, and a transwell invasion assay was used to determine the invasion ability of the two groups. Results: The expression of B7-H3 in bladder urothelial carcinoma was significantly higher than that measured in the adjacent normal tissues (P>0.05). There was no significant difference in cell proliferation between the two groups at 0 hours (P>0.05). From 24 hours, as compared to the control group, the proliferation ability of the B7-H3 low expression group was significantly decreased (P<0.01). Likewise, the invasion ability of the B7-H3 low expression group was significantly decreased (P<0.01) as compared to the control group. Further, as compared to the control group, the expression levels of B7-H3 and MMP-2 in the B7-H3 low expression group were significantly decreased (P<0.01). Conclusion: The interference of shRNA in B7-H3 gene expression can significantly inhibit the proliferation and invasion of bladder urothelial carcinoma T24 cells, implying an important target for the diagnosis and treatment of bladder cancer.

Automated summary

shRNA interference of B7-H3 gene expression can significantly inhibit the proliferation and invasion of bladder urothelial carcinoma T24 cells.