Journal
VETERINARY SCIENCES
Volume 9, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/vetsci9020092
Keywords
CRISPR-Cas9; multiplexed genome editing; zebrafish; tmem183a; hearing loss
Categories
Funding
- National Key Research and Development Program of China [2018YFA0801000]
- Shanghai Center for Brain Science and Brain-Inspired Technology (Shanghai, China)
Ask authors/readers for more resources
This study describes an efficient multiplexed genome editing strategy to generate zebrafish mutants and identified a new candidate gene for hearing loss through behavioral tests and histological examination.
Zebrafish are widely used to investigate candidate genes for human diseases. While the emergence of CRISPR-Cas9 technology has revolutionized gene editing, the use of individual guide RNAs limits the efficiency and application of this technology in functional genetics research. Multiplexed genome editing significantly enhances the efficiency and scope of gene editing. Herein, we describe an efficient multiplexed genome editing strategy to generate zebrafish mutants. Following behavioural tests and histological examination, we identified one new candidate gene (tmem183a) for hearing loss. This study provides a robust genetic platform to quickly obtain zebrafish mutants and to identify candidate genes by phenotypic readouts.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available