Journal
CHEMICAL COMMUNICATIONS
Volume 58, Issue 22, Pages 3633-3636Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1cc05512g
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Funding
- National Research Foundation of Korea (NRF) - Korean government [2021R1A6A1A10039823, 2016R1E1A1A02920873]
- Natural Science Foundation of China [NSFC. 21762045, 21911540466]
- Shandong Provincial Natural Science Foundation [ZR2019YQ12]
- China Postdoctoral Science Foundation [219M652306]
- Taishan Scholar Project [tsqn201812049]
- Korea Basic Science Institute (National Research Facilities and Equipment Center) grant - Ministry of Education [2020R 1A 6C 101B194]
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A coumarin derivative capable of reversibly monitoring glutathione near NMDA receptors was synthesized and found to successfully sense endogenous glutathione in neuronal cells and hippocampal tissues using two-photon fluorescence microscopy. The addition of H2O2 and glutathione induced changes in fluorescence emission, indicating the potential of this probe as an imaging tool.
Glutathione (GSH) is known to play a key role in the modulation of the redox environment in N-methyl-d-aspartate (NMDA) receptors. Coumarin derivative 1 bearing cyanoacrylamide and ifenprodil moieties was synthesized and reported to monitor GSH near NMDA receptors. The cyanoacrylamide moiety allows probe 1 to monitor GSH reversibly at pH 7.4 and the ifenprodil group acts as a directing group for NMDA receptors. Two-photon fluorescence microscopy allows probe 1 to successfully sense endogenous GSH in neuronal cells and hippocampal tissues with excitation at 750 nm. Furthermore, the addition of H2O2 and GSH induced a decrease and an increase in fluorescence emission. Probe 1 can serve as a potential practical imaging tool to get important information on GSH in the brain.
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