Journal
SIGNAL TRANSDUCTION AND TARGETED THERAPY
Volume 7, Issue 1, Pages -Publisher
SPRINGERNATURE
DOI: 10.1038/s41392-022-00881-8
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Funding
- National Key Research and Development Program of China [2016YFC0906000 [2016YFC0906003]]
- National Natural Science Foundation of China [81773752, 81803574, 81902686, 81801980]
- Key Program of the Science and Technology Bureau of Sichuan [2021YFSY0007]
- 1.3.5 project for disciplines of excellence, West China Hospital, Sichuan University [ZYYC20013]
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The study characterized cell types, transcriptional signatures, and differentially expressed genes at each stage of colorectal cancer development using single-cell RNA sequencing, identifying adenoma and carcinoma precursor cell populations and potential drivers of adenoma initiation. The overexpression of these drivers promoted colorectal epithelial cell proliferation and resulted in the formation of polyp-like buds. These findings enhance our understanding of colorectal cancer and may contribute to improved diagnosis and treatment.
The adenoma-carcinoma sequence is a well-accepted roadmap for the development of sporadic colorectal cancer. However, cellular heterogeneity in aberrant epithelial cells limits our understanding of carcinogenesis in colorectal tissues. Here, we performed a single-cell RNA sequencing survey of 54,788 cells from patient-matched tissue samples, including blood, normal tissue, para-cancer, polyp, and colorectal cancer. At each stage of carcinogenesis, we characterized cell types, transcriptional signatures, and differentially expressed genes of distinct cell populations. The molecular signatures of epithelial cells at normal, benign, and malignant stages were defined at the single-cell scale. Adenoma and carcinoma precursor cell populations were identified and characterized followed by validation with large cohort biopsies. Protein tyrosine kinases (PTKs) BMX and HCK were identified as potential drivers of adenoma initiation. Specific BMX and HCK upregulations were observed in adenoma precursor cell populations from normal and adenoma biopsies. Overexpression of BMX and HCK significantly promoted colorectal epithelial cell proliferation. Importantly, in the organoid culture system, BMX and HCK upregulations resulted in the formation of multilayered polyp-like buds protruding towards the organoid lumen, mimicking the pathological polyp morphology often observed in colorectal cancer. Molecular mechanism analysis revealed that upregulation of BMX or HCK activated the JAK-STAT pathway. In conclusion, our work improved the current knowledge regarding colorectal epithelial evolution during carcinogenesis at the single-cell resolution. These findings may lead to improvements in colorectal cancer diagnosis and treatment.
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