4.8 Article

3D Printed Gelatin Scaffold with Improved Shape Fidelity and Cytocompatibility by Using Antheraea pernyi Silk Fibroin Nanofibers

Journal

ADVANCED FIBER MATERIALS
Volume 4, Issue 4, Pages 758-773

Publisher

SPRINGERNATURE
DOI: 10.1007/s42765-022-00135-w

Keywords

Antheraea pernyi silk fibroin nanofiber; 3D printed scaffold; Shape fidelity; Cytocompatibility; Gelatin

Funding

  1. Natural Science Foundation of Shanghai [20ZR1402400]
  2. National Natural Science Foundation of China [52173031, 51903045, 51703033]
  3. Program of Shanghai Academic/Technology Research Leader [20XD1400100]
  4. National Key Research and Development Program of China [2020YFC1910303, 2018YFC1105800]
  5. Basic Research Project of the Science and Technology Commission of Shanghai Municipality [21JC1400100]
  6. Fundamental Research Funds for the Central Universities [2232020D-04, 2232019A3-06, 2232019D3-02]
  7. Science and Technology Commission of Shanghai Municipality [20DZ2254900]

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Combining Antheraea pernyi silk fibroin nanofibers with pure gelatin bioink improves shape fidelity and cytocompatibility in 3D printing, showing promising potential for biomedical applications.
Gelatin (G) is a commonly used natural biomaterial owing to its good biocompatibility and easy availability. However, using pure gelatin as a bioink can barely achieve an ideal shape fidelity in 3D printing. In this study, Antheraea pernyi silk fibroin nanofibers (ASFNFs) with arginine-glycine-aspartic acid (RGD) peptide and partial natural silk structure are extracted and combined with pure gelatin bioink to simultaneously improve the shape fidelity and cytocompatibility of corresponding 3D printed scaffold. Results show that the optimum printing temperature is 30 degrees C for these bioinks. The printed filaments using 16G/4ASFNFs bioink (16wt% gelatin and 4wt% ASFNFs) demonstrate better morphology and larger pore size than those printed by pure gelatin bioink (20G, 20wt% gelatin), thus successfully improve the shape fidelity and porosity of the 3D printed scaffold. The 16G/4ASFNFs scaffold also demonstrate higher swelling ratio and faster degradation rate than the pure gelatin scaffold. Moreover, the cell viability and proliferation ability of Schwann cells cultured on the 16G/4ASFNFs scaffold are significantly superior than those cultured on the pure 20G scaffold. The ASFNFs enhanced 16G/4ASFNFs scaffold reported here are expected to be a candidate with excellent potential for biomedical applications.

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