Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages

Upon viral infection, several proteins in the innate signaling pathway form aggre-gates, which in turn promote the activation of innate antiviral immune response. In this protocol, we use herpes simplex virus type 1 (HSV-1) to infect mouse peri-toneal macrophages, and show how to detect the aggregation of TBK1 upon viral infection. The protocol is adaptable for other proteins and other viruses. For complete details on the use and execution of this profile, please refer to Yan et al. (2021).

Automated summary

This article presents a protocol for detecting the aggregation of TBK1 upon viral infection using HSV-1 infected mouse peritoneal macrophages, which can be adapted for other proteins and viruses.