Journal
STAR PROTOCOLS
Volume 3, Issue 1, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2022.101169
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Funding
- National Foundation of Natural Science of China [31471023, 31800878, 31900736, LZ22C090001]
- Zhejiang Provincial Natural Science Foundation [BX20200297]
- Fellowship of China National Postdoctoral Program for Innovative Talents [P40 OD010440]
- National Institutes of Health-Office of Research Infrastructure Programs
- [31771113]
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We present a detailed protocol for glial Ca2+ imaging in C. elegans, including chemical, mechanical, and optogenetic stimulation approaches, as well as provide detailed instructions for image analysis using Image-J.
Caenorhabditis elegans is an exceptionally transparent model to analyze calcium (Ca2+) signals, but available protocols for neuronal Ca2+ imaging may not be suitable for studying glial cells. Here, we present a detailed protocol for glial Ca2+ imaging in C. elegans following three different approaches including chemical, mechanical, and optogenetic stimulation. We also provide the details for imaging analysis using Image-J.For complete details on the use and execution of this protocol, please refer to Duan et al. (2020).
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