Journal
STAR PROTOCOLS
Volume 3, Issue 1, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2022.101236
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Funding
- German Research Foundation [865634]
- SMA-Europe
- European Research Council Consolidator Grant (ERC CoG)
- NINDS
- NIH Blueprint for Neuroscience Research
- [SI-1969/2-1]
- [SI-1969/3-1]
- [HA6386/10-1]
- [R01-NS078375]
- [R01-AA027079]
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This article describes a new method for analyzing spinal premotor synapses, which enables in-depth study of pathological changes in these synapses during neurodegeneration using confocal and STED imaging, as well as analysis by confocal microscopy.
Loss of synapses on spinal motor neurons is a major feature of several neurode-generative diseases; however, analyzing these premotor synapses is challenging because of their small size and high density. This protocol describes confocal and Stimulated Emission Depletion (STED) imaging of murine spinal premotor synap-ses and their segment-specific quantification by confocal microscopy. We detail the preparation of spinal cord segments, followed by image acquisition and anal-ysis. This protocol enables in-depth analysis of pathological changes in spinal premotor synapses during neurodegeneration.For complete details on the use and execution of this protocol, please refer to Buettner et al. specialIntscript
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