Protocol Generation of highly pure motor neurons from human induced pluripotent stem cells

Editorial Material Cell Biology

Novel insights into the pathogenesis of DYT1 dystonia from induced patient-derived neurons

Baojin Ding

NEURAL REGENERATION RESEARCH (2022)

Article Neurosciences

Disease Modeling with Human Neurons Reveals LMNB1 Dysregulation Underlying DYT1 Dystonia

Baojin Ding et al.

Summary: DYT1 dystonia is a hereditary neurologic movement disorder caused by a mutation in the TOR1A gene encoding an ATPase. Modeling the disease using patient-specific neurons revealed that dysregulation of the nuclear lamina protein LMNB1 could be a major molecular mechanism underlying DYT1 pathology.

JOURNAL OF NEUROSCIENCE (2021)

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Article Cell & Tissue Engineering

Generation of two induced pluripotent stem cell lines with heterozygous and homozygous GAG deletion in TOR1A gene from a healthy hiPSC line

Masuma Akter et al.

Summary: This study successfully generated human induced pluripotent stem cell lines with TOR1A gene GAG deletions through genetic modification, providing a valuable resource in determining the pathogenesis of DYT1 dystonia.

STEM CELL RESEARCH (2021)

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Article Biochemistry & Molecular Biology

Global Transcriptional Analyses of the Wnt-Induced Development of Neural Stem Cells from Human Pluripotent Stem Cells

Bing-Chun Liu et al.

Summary: The study reveals that Wnt signaling plays a crucial role in driving human pluripotent stem cells towards neural stem cells differentiation and maintaining their self-renewal. CLNSCs exhibit spinal cord properties and high proliferative capacity, providing a new cell model for investigating neurogenesis mechanisms and potential applications in regenerative medicine.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

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Article Biochemical Research Methods

Direct conversion of adult fibroblasts into motor neurons

Masood Sepehrimanesh et al.

Summary: Generation of patient-derived neurons offers a novel approach for modeling neurological diseases. Directly converting adult fibroblasts into neurons retains age-related features, making them suitable for modeling age-related neurological diseases. Low yield is a major challenge, requiring high-quality lentiviruses and optimized cell culture conditions.

STAR PROTOCOLS (2021)

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Article Cell Biology