Journal
LAB ON A CHIP
Volume 22, Issue 8, Pages 1438-1468Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1lc01030a
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Funding
- JSPS [20K15151]
- Amada Foundation
- NSG Foundation, Japan
- White Rock Foundation
- Australian Research Council (ARC) [DP200102269]
- Japanese Government (MEXT) Scholarship
- Australian Research Council [DP200102269] Funding Source: Australian Research Council
- Grants-in-Aid for Scientific Research [20K15151] Funding Source: KAKEN
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Single-cell analysis is crucial for understanding the functionality of cells, and single-cell cultivation plays a key role in this process. Microfluidic devices offer efficient and sensitive single-cell cultivation and real-time analysis, with diverse biological applications.
Single-cell analysis is essential to improve our understanding of cell functionality from cellular and subcellular aspects for diagnosis and therapy. Single-cell cultivation is one of the most important processes in single-cell analysis, which allows the monitoring of actual information of individual cells and provides sufficient single-cell clones and cell-derived products for further analysis. The microfluidic device is a fast-rising system that offers efficient, effective, and sensitive single-cell cultivation and real-time single-cell analysis conducted either on-chip or off-chip. Here, we introduce the importance of single-cell cultivation from the aspects of cellular and subcellular studies. We highlight the materials and structures utilized in microfluidic devices for single-cell cultivation. We further discuss biological applications utilizing single-cell cultivation-based microfluidics, such as cellular phenotyping, cell-cell interactions, and omics profiling. Finally, present limitations and future prospects of microfluidics for single-cell cultivation are also discussed.
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