Design, Synthesis and Structure-Activity Relationship Study of Coumarin Benzimidazole Hybrid as Potent Antibacterial and Anticancer Agents

New coumarin-benzimidazole hybrids (3a-f) have been synthesized from 4-formylcoumarins and a series of N-sulphonation (4a-f) and N-methylation (5a-f) compounds were obtained from compounds (3a-f). All the synthesized compounds have exhibited good antimicrobial activity. Docking studies provide valuable insights to potential binding modes of inhibitors. Anti cancer activity of compound 4a and 4c have shown excellent activity against HeLa cell line. Whereas compound 4a and 4d exhibited higher activity against HT 29 cell lines. We calculated the LC50, TGI and GI(50) which show < 10 mg/mL. The synthesized compounds were characterized by IR, NMR, Mass spectral analysis and few of them by single X-ray crystallography analysis.