Phenylboronic acid-functionalized silver nanoparticles for highly efficient and selective bacterial killing

With the widespread use of antibiotics, the number of severe infections caused by unknown pathogens is increasing and novel antibacterial agents with high antibacterial efficiency and selective bacterial killing are urgently needed. In this work, we developed a new kind of functional material based on silver nanoparticles (AgNPs), whose surfaces were functionalized with phenylboronic acid (AgNPs-PBA(n)). The phenylboronic acid groups on the surface of AgNPs-PBA(n) could form covalent bonds with the cis-diol groups of lipopolysaccharide or teichoic acid on the bacterial surface, which highly promoted the interaction between AgNPs-PBA(n) and bacteria, resulting in a very strong enhancement of their antibacterial action via membrane disruption. The scanning electron microscopy images revealed that the accumulation of phenylboronic acid-functionalized AgNPs on the bacterial surface is much more than that of the nonfunctionalized AgNPs. Importantly, the antibacterial efficiency of the phenylboronic acid-functionalized AgNPs on a series of bacteria is 32 times higher than that of bare AgNPs. Moreover, AgNPs-PBA(n) showed a high selectivity toward bacteria with an IC50 (half maximal inhibitory concentration to mammalian cells) more than 160 times its MBC (minimum bactericidal concentration). In a model of an E. coli-infected wound in vivo, AgNPs-PBA(n) could effectively kill the bacteria with an accelerated wound healing rate. This study demonstrates that phenylboronic acid surface functionalization is an efficient way to drastically promote the antibacterial activity of AgNPs, improving the selectivity of silver-based nanoparticles against a variety of bacteria.

Automated summary

This study developed a new functional material based on silver nanoparticles and phenylboronic acid, showing significantly enhanced antibacterial activity by disrupting bacterial membranes and high selectivity against a variety of bacteria.