Journal
ACS SENSORS
Volume 1, Issue 3, Pages 217-221Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.5b00300
Keywords
ricin; aptamer; micromotors; real time-detection; graphene
Funding
- Defense Threat Reduction Agency [HDTRA1-13-1-0002, HDTRA1-14-1-0064]
- CONICYT-CHILE
- Spanish Ministry of Economy and Competitiveness
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An aptamer-based catalytic micromotor sensing strategy for Off-On real-time fluorescent detection of the ricin B toxin is described. This approach relies on self-propelled reduced graphene-oxide (rGO)/platinum (Pt) micromotors, modified with a specific ricin B aptamer tagged with a fluorescein-amidine (FAM) dye, whose fluorescence is quenched due to pi-pi interactions with the rGO surface. The continuous movement of the motor in the sample accelerates the specific binding of the ricin B toxin to the aptamer-dye conjugate and leads to real-time fluorescent On detection. Coupling the Off-On fluorescent switching properties of the aptamer modified-rGO/Pt micromotors with their inherent mixing capabilities thus leads to high speed, simplicity, and sensitivity advantages, thus addressing the limitations of current ricin detection strategies. The new micromotor strategy represents an attractive route for detecting biological threats in a variety of biodefense applications.
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